Molecular Identity Essay Example | Topics and Well Written Essays - 2500 words

molecular Identity - Essay ExampleThe paper, instead, expounds upon the seven cation-coupled chloride cotransporters that have been described to date with specific emphasis fit(p) on KCC2. The cotransporter KCC2 is evident in neuronal processes with specific preference for inhibitory aces where ergic action is relevant. KCC2 action precedes the ergic one as the KCC2 action brings down the intra carrelular chloride concentration to optimum levels to make it possible for ergic action. The phylogenetic, molecular and structural-functional flesh out of the cotransporters have also been provided with special emphasis on KCC2. The CAD cell line, new in studies like this, has been introduced. The cell line exhibits catecholaminergic voltage dependent currents across membranes. Specific experimentation expound have non been provided. A 5708 nucleotide region of the SLC12A5 gene that encodes the KCC2 cotransporter protein was probed with two 20 bp bits, left and right primer details i n Appendix, with RT-PCR analysis. The resulting 206-nucleotide insert was perfectly amplified with one complement in the RT-PCR at temperatures varying from C - C. messenger ribonucleic acid isolated from undifferentiated and differentiated murine CAD cells were used for the analysis. Electrophoresis of the RT-PCR products through 1.5% agarose gel revealed that just about of the inserts had been perfectly complemented once during the RT-PCR producing evidence that the SLC12A5 gene is present in both murine differentiated and undifferentiated CAD cells and that these cells can, thus, later be used as culture media for further study of the KCC2 cotransporter expression and molecular identity. This is of great importance to such study as a suitable cell line has been disfranchised to find so far and the easy manner in which the gene revealed itself in this study assures that this cell line can be a very convenient medium of further such studies limit1. Introduction8-91.1 CAD Cells9- 101.2 The Cotransporters (KCC1, 2, 3 & 4)10-111.3 The Electroneutral Cotransporters11-121.4 Cotransporters KCC111-151.4.1 ancestral Details1.4.2 Structure1.4.3 Function and Location1.5 Cotransporter KCC315-161.5.1 Genetic Details1.5.2 Location1.6 Cotransporter KCC416-171.6.1 Molecular Genetics with Structural Identity1.6.2 Function and Location1.7 Cotransporters General Features171.8 Cotransporter KCC217-241.8.1 Phylogenetics1.8.2 Structural and Locational Implications 1.8.3 Structural and Locational Implications1.8.4 Specific Neuronal Location1.8.5 Regulatory Action1.8.6 Neuron-Specificity1.8.7 Post- and PreNatal Concentration1.8.8 Immunohistochemical epitome2. Methodology 2.1 RNA Isolation Technique242.2 RNA Isolation Protocol24-272.3 Estimation of RNA Yield27-292.3.1 Vol. Isolated RNA in Differentiated Cells2.3.2 Vol. Isolated RNA in Undifferentiated Cells2.4 soil Design292.5 The PCR Analysis29-302.6 Gel Electrophoresis Technique30-313. Results31-414. Discussion42-435. Conclus ion43-44ReferencesBibliographyList of Abbreviations- Chloride anion - gamma aminobutyric acid A type - GABA S type - Potassium cation - Sodium